Evaluation of candidate vaccine approaches for MERS-CoV
Identifieur interne : 001800 ( Main/Exploration ); précédent : 001799; suivant : 001801Evaluation of candidate vaccine approaches for MERS-CoV
Auteurs : Lingshu Wang ; Wei Shi ; M. Gordon Joyce ; Kayvon Modjarrad [États-Unis] ; Yi Zhang ; Kwanyee Leung ; Christopher R. Lees ; Tongqing Zhou ; Hadi M. Yassine ; Masaru Kanekiyo ; Zhi-Yong Yang ; Xuejun Chen ; Michelle M. Becker ; Megan Freeman ; Leatrice Vogel ; Joshua C. Johnson ; Gene Olinger ; John P. Todd ; Ulas Bagci ; Jeffrey Solomon ; Daniel J. Mollura ; Lisa Hensley ; Peter Jahrling ; Mark R. Denison ; Srinivas S. Rao ; Kanta Subbarao ; Peter D. Kwong ; John R. Mascola ; Wing-Pui Kong ; Barney S. GrahamSource :
- Nature Communications [ 2041-1723 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN viral (immunologie), Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Anticorps neutralisants (immunologie), Cellules HEK293, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Femelle, Glycoprotéine de spicule des coronavirus (immunologie), Humains, Immunoglobuline G (immunologie), Infections à coronavirus (), Macaca mulatta, Mâle, Souris, Souris de lignée BALB C, Vaccins antiviraux (immunologie), Vaccins à ADN (immunologie).
- MESH :
- immunologie : ADN viral, Anticorps antiviraux, Anticorps monoclonaux, Anticorps neutralisants, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Immunoglobuline G, Vaccins antiviraux, Vaccins à ADN.
- Animaux, Cellules HEK293, Femelle, Humains, Infections à coronavirus, Macaca mulatta, Mâle, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Coronavirus Infections (prevention & control), DNA, Viral (immunology), Female, HEK293 Cells, Humans, Immunoglobulin G (immunology), Macaca mulatta, Male, Mice, Mice, Inbred BALB C, Middle East Respiratory Syndrome Coronavirus (immunology), Spike Glycoprotein, Coronavirus (immunology), Vaccines, DNA (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, DNA, Viral, Immunoglobulin G, Spike Glycoprotein, Coronavirus, Vaccines, DNA, Viral Vaccines.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- prevention & control : Coronavirus Infections.
- Animals, Female, HEK293 Cells, Humans, Macaca mulatta, Male, Mice, Mice, Inbred BALB C.
Abstract
The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development.
The online version of this article (doi:10.1038/ncomms8712) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1038/ncomms8712
PubMed: 26218507
PubMed Central: 4525294
Affiliations:
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Gordon Joyce</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.420210.5</institution-id><institution-id institution-id-type="ISNI">0000 0001 0036 4726</institution-id><institution>U.S. Military HIV Research Program, Walter Reed Army Institute of Research,</institution></institution-wrap>Silver Spring, 20910 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20910 Maryland USA</wicri:noCountry></affiliation><affiliation wicri:level="2"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.201075.1</institution-id><institution-id institution-id-type="ISNI">0000 0004 0614 9826</institution-id><institution>Henry M. Jackson Foundation for the Advancement of Military Medicine,</institution></institution-wrap>Bethesda, Maryland 20817 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Leung, Kwanyee" sort="Leung, Kwanyee" uniqKey="Leung K" first="Kwanyee" last="Leung">Kwanyee Leung</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Lees, Christopher R" sort="Lees, Christopher R" uniqKey="Lees C" first="Christopher R." last="Lees">Christopher R. 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Denison</name><affiliation><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="GRID">grid.412807.8</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 9916</institution-id><institution>Department of Pediatrics,</institution><institution>Vanderbilt University Medical Center,</institution></institution-wrap>Nashville, 37232 Tennessee USA</nlm:aff><wicri:noCountry code="subfield">37232 Tennessee USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff10"><institution-wrap><institution-id institution-id-type="GRID">grid.412807.8</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 9916</institution-id><institution>Department of Pathology,</institution><institution>Microbiology and Immunology, Vanderbilt University Medical Center,</institution></institution-wrap>Nashville, 37232 Tennessee USA</nlm:aff><wicri:noCountry code="subfield">37232 Tennessee USA</wicri:noCountry></affiliation></author><author><name sortKey="Rao, Srinivas S" sort="Rao, Srinivas S" uniqKey="Rao S" first="Srinivas S." last="Rao">Srinivas S. Rao</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name><affiliation><nlm:aff id="Aff6"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Emerging Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Kwong, Peter D" sort="Kwong, Peter D" uniqKey="Kwong P" first="Peter D." last="Kwong">Peter D. Kwong</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Mascola, John R" sort="Mascola, John R" uniqKey="Mascola J" first="John R." last="Mascola">John R. Mascola</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author><author><name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S." last="Graham">Barney S. Graham</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.419681.3</institution-id><institution-id institution-id-type="ISNI">0000 0001 2164 9667</institution-id><institution>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health,</institution></institution-wrap>Bethesda, 20892 Maryland USA</nlm:aff><wicri:noCountry code="subfield">20892 Maryland USA</wicri:noCountry></affiliation></author></analytic><series><title level="j">Nature Communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Coronavirus Infections (prevention & control)</term><term>DNA, Viral (immunology)</term><term>Female</term><term>HEK293 Cells</term><term>Humans</term><term>Immunoglobulin G (immunology)</term><term>Macaca mulatta</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Vaccines, DNA (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (immunologie)</term><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Cellules HEK293</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Humains</term><term>Immunoglobuline G (immunologie)</term><term>Infections à coronavirus ()</term><term>Macaca mulatta</term><term>Mâle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins à ADN (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>DNA, Viral</term><term>Immunoglobulin G</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN viral</term><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Anticorps neutralisants</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Immunoglobuline G</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>HEK293 Cells</term><term>Humans</term><term>Macaca mulatta</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HEK293</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus</term><term>Macaca mulatta</term><term>Mâle</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/ncomms8712) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Assiri, A" uniqKey="Assiri A">A Assiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Breban, R" uniqKey="Breban R">R Breban</name></author><author><name sortKey="Riou, J" uniqKey="Riou J">J Riou</name></author><author><name sortKey="Fontanet, A" uniqKey="Fontanet A">A Fontanet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cauchemez, S" uniqKey="Cauchemez S">S Cauchemez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Graham, Rl" uniqKey="Graham R">RL Graham</name></author><author><name sortKey="Donaldson, Ef" uniqKey="Donaldson E">EF Donaldson</name></author><author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buchholz, Uj" uniqKey="Buchholz U">UJ Buchholz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coleman, Cm" uniqKey="Coleman C">CM Coleman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, C" uniqKey="Ma C">C Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mou, H" uniqKey="Mou H">H Mou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ohnuma, K" uniqKey="Ohnuma K">K Ohnuma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ying, T" uniqKey="Ying T">T Ying</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, L" uniqKey="Jiang L">L Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin, Je" uniqKey="Martin J">JE Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Naldini, L" uniqKey="Naldini L">L Naldini</name></author><author><name sortKey="Blomer, U" uniqKey="Blomer U">U Blomer</name></author><author><name sortKey="Gage, Fh" uniqKey="Gage F">FH Gage</name></author><author><name sortKey="Trono, D" uniqKey="Trono D">D Trono</name></author><author><name sortKey="Verma, Im" uniqKey="Verma I">IM Verma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stevens, Tl" uniqKey="Stevens T">TL Stevens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davis, Hl" uniqKey="Davis H">HL Davis</name></author><author><name sortKey="Schirmbeck, R" uniqKey="Schirmbeck R">R Schirmbeck</name></author><author><name sortKey="Reimann, J" uniqKey="Reimann J">J Reimann</name></author><author><name sortKey="Whalen, Rg" uniqKey="Whalen R">RG Whalen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shedlock, Dj" uniqKey="Shedlock D">DJ Shedlock</name></author><author><name sortKey="Weiner, Db" uniqKey="Weiner D">DB Weiner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cargnelutti, De" uniqKey="Cargnelutti D">DE Cargnelutti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chaitra, Mg" uniqKey="Chaitra M">MG Chaitra</name></author><author><name sortKey="Nayak, R" uniqKey="Nayak R">R Nayak</name></author><author><name sortKey="Shaila, Ms" uniqKey="Shaila M">MS Shaila</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prabakaran, P" uniqKey="Prabakaran P">P Prabakaran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mansoor, A" uniqKey="Mansoor A">A Mansoor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, Xc" uniqKey="Tang X">XC Tang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caulfield, Mj" uniqKey="Caulfield M">MJ Caulfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kong, Wp" uniqKey="Kong W">WP Kong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ledgerwood, Je" uniqKey="Ledgerwood J">JE Ledgerwood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ledgerwood, Je" uniqKey="Ledgerwood J">JE Ledgerwood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khurana, S" uniqKey="Khurana S">S Khurana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J Sui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mclellan, Js" uniqKey="Mclellan J">JS McLellan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mclellan, Js" uniqKey="Mclellan J">JS McLellan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barouch, Dh" uniqKey="Barouch D">DH Barouch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Catanzaro, At" uniqKey="Catanzaro A">AT Catanzaro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reed, Lj" uniqKey="Reed L">LJ Reed</name></author><author><name sortKey="Muench, H" uniqKey="Muench H">H Muench</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otwinowski, Z" uniqKey="Otwinowski Z">Z Otwinowski</name></author><author><name sortKey="Minor, W" uniqKey="Minor W">W Minor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccoy, Aj" uniqKey="Mccoy A">AJ McCoy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P Emsley</name></author><author><name sortKey="Lohkamp, B" uniqKey="Lohkamp B">B Lohkamp</name></author><author><name sortKey="Scott, Wg" uniqKey="Scott W">WG Scott</name></author><author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blanc, E" uniqKey="Blanc E">E Blanc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, Zc" uniqKey="Fan Z">ZC Fan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pak, Je" uniqKey="Pak J">JE Pak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adams, Pd" uniqKey="Adams P">PD Adams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Vb" uniqKey="Chen V">VB Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scobey, T" uniqKey="Scobey T">T Scobey</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Bagci, Ulas" sort="Bagci, Ulas" uniqKey="Bagci U" first="Ulas" last="Bagci">Ulas Bagci</name><name sortKey="Becker, Michelle M" sort="Becker, Michelle M" uniqKey="Becker M" first="Michelle M." last="Becker">Michelle M. Becker</name><name sortKey="Chen, Xuejun" sort="Chen, Xuejun" uniqKey="Chen X" first="Xuejun" last="Chen">Xuejun Chen</name><name sortKey="Denison, Mark R" sort="Denison, Mark R" uniqKey="Denison M" first="Mark R." last="Denison">Mark R. Denison</name><name sortKey="Freeman, Megan" sort="Freeman, Megan" uniqKey="Freeman M" first="Megan" last="Freeman">Megan Freeman</name><name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S." last="Graham">Barney S. Graham</name><name sortKey="Hensley, Lisa" sort="Hensley, Lisa" uniqKey="Hensley L" first="Lisa" last="Hensley">Lisa Hensley</name><name sortKey="Jahrling, Peter" sort="Jahrling, Peter" uniqKey="Jahrling P" first="Peter" last="Jahrling">Peter Jahrling</name><name sortKey="Johnson, Joshua C" sort="Johnson, Joshua C" uniqKey="Johnson J" first="Joshua C." last="Johnson">Joshua C. Johnson</name><name sortKey="Joyce, M Gordon" sort="Joyce, M Gordon" uniqKey="Joyce M" first="M. Gordon" last="Joyce">M. Gordon Joyce</name><name sortKey="Kanekiyo, Masaru" sort="Kanekiyo, Masaru" uniqKey="Kanekiyo M" first="Masaru" last="Kanekiyo">Masaru Kanekiyo</name><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name><name sortKey="Kwong, Peter D" sort="Kwong, Peter D" uniqKey="Kwong P" first="Peter D." last="Kwong">Peter D. Kwong</name><name sortKey="Lees, Christopher R" sort="Lees, Christopher R" uniqKey="Lees C" first="Christopher R." last="Lees">Christopher R. Lees</name><name sortKey="Leung, Kwanyee" sort="Leung, Kwanyee" uniqKey="Leung K" first="Kwanyee" last="Leung">Kwanyee Leung</name><name sortKey="Mascola, John R" sort="Mascola, John R" uniqKey="Mascola J" first="John R." last="Mascola">John R. Mascola</name><name sortKey="Mollura, Daniel J" sort="Mollura, Daniel J" uniqKey="Mollura D" first="Daniel J." last="Mollura">Daniel J. Mollura</name><name sortKey="Olinger, Gene" sort="Olinger, Gene" uniqKey="Olinger G" first="Gene" last="Olinger">Gene Olinger</name><name sortKey="Rao, Srinivas S" sort="Rao, Srinivas S" uniqKey="Rao S" first="Srinivas S." last="Rao">Srinivas S. Rao</name><name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name><name sortKey="Solomon, Jeffrey" sort="Solomon, Jeffrey" uniqKey="Solomon J" first="Jeffrey" last="Solomon">Jeffrey Solomon</name><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name><name sortKey="Todd, John P" sort="Todd, John P" uniqKey="Todd J" first="John P." last="Todd">John P. Todd</name><name sortKey="Vogel, Leatrice" sort="Vogel, Leatrice" uniqKey="Vogel L" first="Leatrice" last="Vogel">Leatrice Vogel</name><name sortKey="Wang, Lingshu" sort="Wang, Lingshu" uniqKey="Wang L" first="Lingshu" last="Wang">Lingshu Wang</name><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name><name sortKey="Yassine, Hadi M" sort="Yassine, Hadi M" uniqKey="Yassine H" first="Hadi M." last="Yassine">Hadi M. Yassine</name><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name><name sortKey="Zhou, Tongqing" sort="Zhou, Tongqing" uniqKey="Zhou T" first="Tongqing" last="Zhou">Tongqing Zhou</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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